The Impact of Extract from Astragalus membranaceus on Blood Coagulation in Individuals with Diabetes Mellitus

Authors

  • Duangmanee Sanmun Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Chatchawan Changtam Division of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Soramon Sutin Division of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Kanjana Sirirat Division of Transfusion Science, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Nonthaya Tangrua Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Nopphadet Manoowong Department of Medical Technology and Clinical Pathology, Samutsakhon Hospital, Samut Sakhon, 74000 Thailand
  • Yupha Arsarach Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Korawan Worakunchanakit Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Natnicha Thipphasorn Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Sasivimol Khunnen Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Nattarika Suwannakam Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand
  • Watcharapon Phengrueang Division of Hematology and Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540 Thailand

Keywords:

Astragalus, Coagulation, Diabetes mellitus, Hemostasis

Abstract

Diabetes-related vascular problems might result from persistent hyperglycemia, and individuals with diabetes have an increased risk of blood clot formation compared to healthy controls. Anticoagulant drugs are medications used to dissolve blood clots; various agents have been employed to improve platelet function and minimize thrombosis in these patients. Medicinal plants can be used as alternatives with potentially fewer side effects. Interestingly, Astragalus membranaceus (also known as Huangqi) extract was obtained for in vitro investigation. This study aimed to assess the anticoagulant impact of Huangqi on human plasma. The research focused on prothrombin time (PT) and activated partial thromboplastin time (aPTT) among diabetic patients. In vitro anticoagulant effects were evaluated using plasma treated with the extract, while DMSO served as a negative control. The results showed that the baseline PT in untreated plasma from the healthy group was 11.79 seconds, compared to 11.69 seconds in the diabetic group. A significant difference (P<0.05) was observed between healthy donors and diabetic patients; the mean aPTT in diabetic patients was 26.03 seconds, while in controls, it was 28.52 seconds. The present study highlighted the potent antithrombin effect of Huangqi extract at a dosage of 50 mg/ml. The PT and aPTT assays were significantly prolonged by Huangqi (P<0.001), demonstrating effective anticoagulant activity. This suggests that Huangqi may benefit patients who are at high risk of thrombosis.

References

Abdulrahaman, Y., & Dallatu, M. K. (2012). Evaluation of Prothrombin Time and Activated Partial Thromboplastin in Patients with Diabetes Mellitus. Nigerian Journal of Basic and Applied Science, 20(1), 60-63.

Boshabor, S. O. (2022). Prothrombin time, activated partial thromboplastin time and platelet count in patients with diabetes mellitus. MINAR International Journal of Applied Sciences and Technology, 04(02):186-195.

Cai, C., Gao, S., Wang, B., Zhao, T., Gao, H., Shi, Y., Guo, X., Xu, Y., Sun, Y., Guo, D., Zhang, J., Gao, F., Liu, Q., Fu, Z., Zhu, L., Liu, X., Wang, L., & Zhao, Y. (2025). Astragaloside IV as a potent vWbp inhibitor: An innovative strategy to mitigate foodborne Staphylococcus aureus infections. Biochemical Pharmacology, 239, 117017. https://doi.org/10.1016/j.bcp.2025.117017

Chen, M., Fu, B., Zhou, H., & Wu, Q. (2025). Therapeutic potential and mechanistic insights of astragaloside IV in the treatment of arrhythmia: a comprehensive review. Frontiers in Pharmacology, 16, 1528208. https://doi.org/10.3389/fphar.2025.

Danthaiwattana, S. Pojpanichphong, S. & Solod, W. (2021). Roles of Chinese Medicine: Huangqi (黄芪: Astragalus) in the Prevention and Treatment of Coronavirus Disease 2019. Regional Health Promotion Center 9 Journal, 15(37), 365-76. (in Thai)

Guo, Y., Yang, P., Wu, Z., Zhang, S., & You, F. (2025). Mechanisms of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (huang qi) and Angelica sinensis (Oliv.) Diels (dang gui) in Ameliorating Hypoxia and Angiogenesis to Delay Pulmonary Nodule Malignant Transformation. Integrative cancer therapies, 24, 15347354241311917. https://doi.org/10.1177/15347354241311917

Janwitayanuchit, I., Semsri, S., Janwitayanuchit, W., & Choowongkomon, K. (2024). Effect of mangiferin isolated from Mangifera indica leaves on in vitro blood coagulation and cell migration activities. Journal of Associated Medical Sciences, 57(2), 67–75. retrieved from https://he01.tci-thaijo.org/index.php/bulletinAMS/article/view/267475

Kaur, R., Kaur, M., & Singh, J. (2018). Endothelial dysfunction and platelet hyperactivity in type 2 diabetes mellitus: molecular insights and therapeutic strategies. Cardiovascular diabetology, 17(1), 121. https://doi.org/10.1186/s12933-018-0763-3

Kim, J. H. (2012). Studies on the biological activity of Astragalus membranaceus extracts. Biomedical Science Letters, 18(1), 35-41.

Law, S., Lo, C. H. U. I. M. A. N., Han, J., Leung, A. W., & Xu, C. (2020). Traditional Chinese herb, Astragalus: possible for treatment and prevention of COVID-19?. Herba Polonica, 66(4).

Liao, H., Hu, L., Cheng, X., Wang, X., Li, J., Banbury, L., & Li, R. (2017). Are the therapeutic effects of Huangqi (Astragalus membranaceus) on diabetic nephropathy correlated with its regulation of macrophage iNOS activity?. Journal of Immunology Research, 2017(1), 3780572. https://doi.org/10.1155/2017/3780572

Li, X., Weber, N. C., Cohn, D. M., Hollmann, M. W., DeVries, J. H., Hermanides, J., & Preckel, B. (2021). Effects of Hyperglycemia and Diabetes Mellitus on Coagulation and Hemostasis. Journal of Clinical Medicine, 10(11), 2419. https://doi.org/10.3390/jcm10112419

Li, X., Qu, L., Dong, Y., Han, L., Liu, E., Fang, S., & Wang, T. (2014). A review of recent research progress on the astragalus genus. Molecules, 19(11), 18850-18880. https://doi.org/10.3390/molecules191118850

Liu, C., Zhang, J., Mao, K., Xu, H., & He, Y. (2025). Astragalus membranaceus-Carthamus tinctorius herb pair antagonizes parthanatos in cerebral ischemia/reperfusion injury via regulating PARP-1/TAX1BP1-mediated mitochondrial respiratory chain complex I. Journal of Ethnopharmacology, 340, 119260. https://doi.org/10.1016/j.jep.2024.119260

Liu, S., Wang, L., Zhang, Z., Leng, Y., Yang, Y., Fu, X., Xie, H., Gao, H., &Xie, C. (2024), The potential of astragalus polysaccharide for treating diabetes and its action mechanism. Frontiers in Pharmacology, 15, 1339406. https://doi.org/10.3389/fphar.2024.1339406

Liu, M., Wu, K., Mao, X., Wu, Y., & Ouyang, J. (2010). Astragalus polysaccharide improves insulin sensitivity in KKAy mice: regulation of PKB/GLUT4 signaling in skeletal muscle. Journal of ethnopharmacology, 127(1), 32-37.

Ma, X. Q., Shi, Q., Duan, J. A., Dong, T. T., & Tsim, K. W. (2002). Chemical analysis of Radix Astragali (Huangqi) in China: a comparison with its adulterants and seasonal variations. Journal of agricultural and food chemistry, 50(17), 4861-4866. https://doi.org/10.1021/jf0202279

Maschirow, L,. Khalaf, K., & Al-Aubaidy, et al. (2015). Inflammation, coagulation, endothelial dysfunction and oxidative stress in prediabetes — Biomarkers as a possible tool for early disease detection for rural screening. Clinical Biochemistry, 48(9), 581-585. https://doi.org/10.1016/j.clinbiochem.2015.02.015.

Mazhar, M. U., Anwar, F., Saleem, U., Ahmad, B., Mirza, M. U., & Ahmad, S. (2022). Antiplatelet and anticoagulant activities of Astragalus sarcocolla Dymock. Pharmacognosy Magazine, 18(80).

Mazhar, M. U., Muneer, K., Anwar, F., Siddiqui, W. A., Qureshi, A. Q., Bilal, R., & Ahmad, B. (2020). In-vitro anticoagulant activity of three extracts of Astragalussarcocolla. Pakistan J Med Heal Sci, 14(2), 393-6.

Sabeena, S., Reddy, K. S., Banu, K. F., Ramya, N., Sudheer, A., & Chidrawar, V. R. (2022). Evaluation of Anti-Thrombotic Activity of Astragalus Membranaceus Var. Mongholicus) Root Extract: In Vitro. Neuroquantology, 20(17), 1020-1024. https://doi.org/10.14704/Nq.2022.20.17.Nq880130

Thippayacharoentam, C., Narajeenrone, K., Chaowpeerapong, T., Wannachalee, T., & Akarasereenont, P. (2022). Management of Diabetic Patients with Thai Traditional Medicine. Siriraj Medical Bulletin, 15(3), 181-188.

Tang, X., Liu, X., Mikaye, M. S., Hongrui, Z,. & Zhang, Yong. (2021). Traditional Chinese medicine in the treatment of high incidence diseases in cold areas: the thrombotic diseases. Frigid Zone Medicine, 1(1), 23-44. http://doi:.org/10.2478/fzm-2021-0005

Tripatara, A., & Chumpia, W. (2004). Hemostasis testing (2nd ed.). Khon Kaen University Press. (in Thai)

Xiang, T., Wang, L.R., Ma, L., Han, Z. Y., Wang, R., Cheng, C., Xia, Y., Qin, H., & Zhao, C. S. (2014). From Commodity Polymers to Functional Polymers. Scientific Reports, 4(1), 4604. https://doi.org/10.1038/srep04604

Zhang, J., Wu, C., Gao, L., Du, G., & Qin, X. (2020). Astragaloside IV derived from Astragalus membranaceus: A research review on the pharmacological effects. Advances in pharmacology (San Diego, Calif.), 87, 89–112. https://doi.org/10.1016/bs.apha.2019.08.002

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Published

2025-08-14

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Vol. 24 No. 2 (2025): May - August

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Research Articles

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