Influence of Fruit Juices and Optimum of Ammonium Sulfate on the Bacterial Cellulose Production

Abstract

Bacterial cellulose is a product obtained from the synthesis of cellulose outside the cell, produced by bacteria. The purpose of this research is to study the influence of fruit juices and the optimum of ammonium sulfate on bacterial cellulose production from Acetobacter xylinum. The experiments were conducted by a 2 × 3 factorial experiment using a completely randomized design with five replications. There were two types of fruit juice, including coconut water and pineapple juice. Three levels of ammonium sulfate were used at 0.25%, 0.50% and 0.75%. Wet weight and thickness of bacterial cellulose sheet were collected. The results showed that there was a significant difference (p < 0.05) between the main effects of fruit juices and the amount of ammonium sulfate on the wet weight and thickness of bacterial cellulose. However, there was no interaction effect between them. The treatments that gave the highest average of wet weight and thickness were coconut water (wet weight 37.671.94 g., thickness 2.740.18 cm.) and the concentration levels 0.25% or 0.50% of ammonium sulfate (wet weight 29.689.46 and 30.009.08 g. respectively, thickness 2.150.73 and 2.180.71 cm. respectively)