Direct androgenesis in anther culture of butterfly pea related to flower development

Authors

  • Kitti Bodhipadma Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok
  • Sompoch Noichinda Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok
  • Kridsana Onnui Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok
  • Saowaros Phanomchai Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok
  • Luepol Punnakanta Faculty of Environment and Resource Studies, Mahidol University
  • David W.M. Leung Division of Agro-Industrial Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok

Keywords:

Direct androgenesis in anther culture of butterfly pea related to flower development

Abstract

Androgenesis is a biotechnological pathway of in vitro gametic embryogenesis with the potential leading to haploid plant production from male reproductive organ. Butterfly pea (Clitoria ternatea L.) is a versatile leguminous plant with the floral shape resembling a butterfly at full bloom. Based on the varying lengths of flower as well as petals and sepals, 5 stages of flower development were delineated. When anthers from flowers of these various stages were cultured on basal Murashige and Skoog medium supplemented with 3 mg/l 2,4-D and 60 or 80 g/l sucrose, it was found that anther-derived embryos (11.67 and 3.3% in the presence of 6 and 8% sucrose, respectively) were obtained only from stage 2 flowers. Light microscopic observation also revealed that the microspores of anthers isolated from flowers at stage 2 was uninucleate. This suggested that anther or microspore stage of butterfly pea played a very important role in direct androgenesis and 8% sucrose in the medium was detrimental to this process.

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Published

2017-06-01

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Section

Research Articles