Simultaneous extraction of oil and protein from perilla seed by three-phase partitioning and their application in serum

Abstract

Perilla seeds contain high amount of oil and protein. This study was aimed to simultaneously extract oil and protein from perilla seed by using three-phase partitioning method (TPP). The extraction conditions were optimized by varying the ratio of crude extract to t-butanol of 1:2, 1:1 and 2:1 (v/v) and ammonium sulfate concentration of 20, 30 and 40% (w/v). The crude extract to t-butanol at 1:2 and 30% (w/v) ammonium sulfate resulted in the highest recovery of perilla oil (35.13±2.67%) and protein (13.18±0.65%). This provided 14 folds of time saving when compared to conventional method of oil and protein extraction separately. The obtained perilla protein and oil extracts were included in the facial serum formulation. Stability and satisfaction of the serum formulation were tested compared to its base formula. The perilla facial serum showed its good stability after accelerated conditions of centrifugation and heating-cooling for 4 cycles. The phase separation and pH changing was not observed. The viscosity and the color were slightly affected by the accelerated condition. The satisfaction test including moisturizing sensory of the serum was evaluated by 20 volunteers. The product was satisfied with average score 5.47 of the maximum 6 level. This study emphasized the valuable perilla seed protein and oil could be extracted by single step of TPP and feasibly included into moisturizing facial serum product.