Anti-aging efficacy of Thai red rice callus cosmetic product

Abstract

Plant cell culture is an innovative technology to produce bioactive substances and antiaging agents for cosmetic products. Red rice (Oryza sativa) is a special rice cultivar in Thailand that has high procyanidin content in seed coats. In this study, the red rice callus were cultured from rice seeds and extracted with ethanol. The extract contained 0.42 mg GAE/ml for phenolic compounds and 0.21 mg Glu/ml for amino acids. The anti-aging properties of the induced rice callus extract in solution form was evaluated on 28 volunteers, aged 30 – 55 years. The volunteers were separated into 3 groups; placebo (n = 8), 5 % of the induced stem cell extract cream (n = 10), and 5 % of the commercial red rice stem cell extract cream (n = 10). The tested creams were applied on facial skin twice a day for 12 weeks. Skin color, hydration and elasticity were assessed with Mexameter, Corneometer, and Cutometer, respectively. The results showed that anti-aging efficacies of the group treated red rice stem cell extract cream were greater than those of the commercial cream group, which were skin whitening (38.57%, 29.85%), skin moisture (74.62%, 33.48%) and skin elasticity (88.41%, 64.97%), respectively, however, the placebo group remained no change of skin in any parameters. In sensory evaluation, most of volunteers satisfied with product acceptability; appearance, texture, color, odor, moisture, spreadibility, absorption, smoothness, non-greasiness (> 85 %) and also the reduction of visible aging sign (> 90%). The results demonstrate that Thai red rice stem cell extract can be used as an effective anti-aging ingredient for cosmetic products functionalized in skin lightening, hydration and elasticity.