Transesterification reaction for palm based wax esters by immobilized lipase EQ3 isolated from wastewater of fish canning industry

Abstract

Palm olein based wax esters were synthesized by tranesterification for increasing value of palm olein and applications as well as reducing chemical usage. The immobilized lipase EQ3 from Burkholderia sp. EQ3 which was isolated from wastewater of fish canning factory was used as biocatalyst in liquid wax esters synthesis reaction. The optimal reaction conditions for palm olein based wax esters synthesis were studied for immobilized lipase EQ3 dosages, temperatures, the molar ratios of palm olein to oleyl alcohol, substrate concentrations and organic solvents. All of these factors had an effect on efficiencies of enzymatic transesterification reaction. The optimal conditions were 10 U of immobilized lipase loading, temperature of 37°C, the molar ratio of palm olein to oleyl alcohol at 1:3 (mol/mol) and the substrate concentration of 0.33% (w/v) in isooctane, and 12 h of the reaction time producing the palm olein based wax esters reached the highest synthesis more than 80%.