Quantitative analysis of total selenium in of Se-enriched yeast products with ICP-MS using home-made closed digestion system for sample preparation
Authors
- Witchapol Thosaikham Lecturer
Keywords:
Selenium, closed-digestion system, Se-enriched yeast, water bath, wet acid digestionAbstract
Sample digestion is an important procedure and directly affects on the reliability of quantitative analysis of total selenium in Se-enriched yeast products. The home-digestion closed digestion system was successfully developed by specially designing and constructing from the basic equipments in laboratory that consisted of 4 main devices; water bath, cover plate, vessels and wind tunnel. The optimum conditions of the home-made closed digestion system was accurately and precisely achieved by using 2.0 ml of nitric acid for 0.1 g Se-enriched yeast sample, 100 °C digestion temperature and 20 min digestion time. These conditions showed good validation and acceptation with the certified value of Se-enriched yeast reference material. Furthermore, the digestion efficiency of home-made closed digestion system was as well as the recognized digestion methods especially microwave assisted digestion. Consequently, the home-made closed digestion system could be efficiently utilized as an alternative digestion method for total Se determination in Se-enriched yeast products. Additionally, the home-made closed digestion system was adapted from a basic instrument in laboratory which has affordability and easier operation rather than the recognized methods.
Author Biography
Witchapol Thosaikham, Lecturer
CURRICULUM VITAE
WITCHAPOL THOSAIKHAM
ORCID ID: 0000-0002-8159-3789
AFILIATION
Dapartment of Chemistry, Faculty of Science and Technology, Sakon Nakhon Ratjabhat University, Sakon Nakhon 47000
EDUCATION
Ph.D in Chemistry 2016
Mahasarakham University, Thailand
M.Sc. in Chemistry 2010
Mahasarakham University, Thailand
B.Sc. in Chemistry 2007
Mahasarakham University, Thailand
FELLOWSHIP AND AWARS
Research grant for Ph.D. dissertation 2013-2015
Division of Research Facilitation and Dissemination, Mahasarakham University
Bhumibol Scholarship 2013
Research grant for Master thesis 2008-2010
Division of Research Facilitation and Dissemination, Mahasarakham University
Scholarship for Master Degree 2007-2010
Center of Excellence for Innovation in Chemistry (PERCH-CIC), Thailand
Research grant for Senior Project of the 4th year undergraduate student 2006
Division of Research Facilitation and Dissemination, Mahasarakham University
RESEARCH EXPERIENCES
- Development of Se-enriched probiotic cultivation and Se speciation analysis 2011 - 2015
- Design and construction home-made electrodialysis and application to removing 2011 - 2015
SeO32- ion from remained-medium of Se-enriched probiotics
- Development of home-made closed vessel digestion and application to preparation 2008 - 2012
of biological samples prior to determination of trace elements contents by AAS and ICP-MS
- Se-enriched plant production, Se speciation analysis, and application to poultry diet 2007 – 2010
- Quantitative determination of selenium contents in plasma of laying hen using 2006 – 2007
ICP-MS Technique
RESEARCH INTERESTS
- Se-enriched food production and application for producing Se-enriched food with potential to be used in the pharmaceutical or food industry.
- Development of elemental speciation analysis and metallomics
- Development of sample introduction system for atomic spectroscopy techniques such as FAAS, HG-AAS, GF-AAS, ICP-OES and ICP-MS
- Development of sample preparation techniques such as green extraction, digestion and drying
- Development sample preparation and analytical method for GC, GC-MS and HPLC
ANAYTICAL TECHNIQUE SKIL
ICP-OES, ICP-MS, LC-ICP-MS, AAS, HG-AAS, HPLC, GC, UV-Visible spectrometry, Polarimeter, Reflectometer and IR spectrometry
WORK EXPERIENCES
Erawan Sugar Company Limited, Nong Bua Lamphu Province, Thailand 2016 - 2017
- Head of Chemical Analysis Center and Quality Control
Program of Chemistry, Nakhon Ratchasima Rajabhat University, Thailand
- Special Instructor, Principal Chemistry 1 and Chemistry Laboratory 1, 1st semester, 2013
Ministry of Science and Technology of Thailand
- Data Collector, Project Science and Technology Infrastructure Databank (STDB) April – October 2011
Department of Chemistry, Mahasarakham University, Thailand
- Assistant Teaching, Analytical Chemistry Laboratory 2 2006 - 2008
- Assistant Teaching, General Chemistry Laboratory 1 2007- 2009
- Assistant Teaching, Analytical Chemistry 2, Topic AAS, 2nd semester, 2015
PATENTS
Thosaikham W, Sujira Maneerat and Chantiratikul P, Process of Se-enriched biomass from Lactobacillus, Thai Patent Application No. 1501004987. Bangkok: Department of Intellectual Property, 2016
Thosaikham W and Chantiratikul P, Electrodialysis Apparatus for Protein Separation, Thai Patent No. 11624. Bangkok: Department of Intellectual Property, 2016
Thosaikham W, Sittipout R and Chantiratikul P (2011) Thai Patent No. 6432. Bangkok: Department of Intellectual Property.
PUBPLICATIONS
Chantiratikul A, Borisuth L, Chinrasri O, Saenthaweesuk N, Chookhampaeng S, Thosaikham W, Sriart N, Chantiratikul P, Evaluation of the toxicity of selenium from hydroponically produced selenium-enriched kale sprout in laying hens, J. Trace Elem. Med. Biol. Vol. 35, pp. 116-121, 2016
10
Thosaikham W, Jitmanee K, Sittipout R, Maneetong S, Chantiratikul A and Chantiratikul P (2014) Evaluation of selenium species in selenium-enriched pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee) using mixed ion-pair reversed phase HPLC-ICP-MS. Food Chemistry, 145, 736-42.
Maneetong S, Chookhampaeng S, Chantiratikul A, Chinrasri O, Thosaikham W, Sittipout R and Chantiratikul P (2013) Hydroponic cultivation of selenium-enriched kale (Brassica oleracea var. alboglabra L.) seedling and speciation of selenium with HPLC–ICP-MS. Microchemical Journal, 108:87-91.
Chantiratikul A, Chinrasri O, Pakmaruek P, Chantiratikul P, Thosaikham W and Sittipout R (2012) Responses of Growing Japanese Quails that Received Selenium from Selenium-Enriched Kale Sprout (Brassica oleracea var. alboglabra L.). Biological Trace Element Research, 144, 760-768.
Chantiratikul A, Chinrasri O, Chantiratikul P, Thosaikham W, Maneethong S and Aengwanich W (2011) Effect of Selenium from Selenium-Enriched Kale Sprout (Brassica oleracea var. alboglabra L.) on Selenium Concentration in Tissue of Spent Hens. Khon Kean Agricultural Journal, 40(2), 295-298.
Chinrasri O, Chantiratikul P, Thosaikham W, Atiwetin P, Chumpawadee S, Saenthaweesuk S and Chantiratikul A (2009) Effect of Selenium-enriched Bean Sprout and Other Selenium Sources on Productivity and Selenium Concentration in Eggs of Laying Hens. Asian-Australasian Journal of Animal Sciences, 22(12), 1661-1666
Thosaikham W and Chantiratikul P (2008) Application of HPLC-ICP-MS technique on elemental speciation analysis. Naresuan Phayou Journal, 3(1), 154-163.
PROCEEDING
Thosaikham W, Maneerat S, Chantiratikul P (2017) Evaluation of extracellular selenium speciation in Lactobacillus. Journal of Trace Element in Medicine and Biology, Volume 41S1, p. 61
Chantiratikul P, Thosaikham W, Maneerat S, (2017) Cultivation of Se-enriched L. casei subsp. rhamnosus for using as Se-enriched diet, Journal of Trace Element in Medicine and Biology, Volume 41S1, p. 60
Borisuth L, Saenthaweesuk N, Chinrasri O, Thosaikham W, Chantiratikul P, Sriart N, and Chantiratikul A (2015) Toxicity of Se from Se-enriched kale sprout (Brassica oleracea var. albogiabla L.) on performance and egg quality in laying hens. Mahasarakham University Science and Technology Journal, Special volume, 270-275.
Thosaikham W, Maneethong S, Sittipout R and Chantiratikul P (2012) Extraction Method for extracting Selenium from Se-enriched Plants. Mahasarakham University Science and Technology Journal, Special volume, 146-151
Sittipout R, Thosaikham W, Chantiratikul P and Burakham R (2011) Development of Home-made Digestion Apparatus for Determination of Iron and Zinc with Flame Atomic Spectrometry Technique in Biological Samples. Mahasarakham University Science and Technology Journal, Special volume, 74-79.
Thosaikham W, Sittipout R and Chantiratikul A (2009) Sample preparation methods for selenium determination with ICP-MS technique. Mahasarakham University Science and Technology Journal. Special volume, 71-78.
CONFERENCE
Oral presentation
Thosaikham W, Maneerat S, Chantiratikul P. Application of Ion-paired reversed phase HPLC-ICP-MS for Determination of Extracellular Selenium Speciation in Selenium-enriched L. rhamnosus culture. European Symposium on Atomic Spectrometry 2016 (ESAS 2016), 31 March – 2 April 2016, Eger, Hungary
Thosaikham W, Sittipout R and Chantiratikul P (2014) Modification of Water Bath as a Newly Digestion Apparatus and Application for Preparation of Mushroom Samples Prior to Determine Fe Concentration by AAS. Pure and Applied Chemistry International Conference (PACCON 2014). 8 - 10 January 2014, Khon Kean, Thailand.
Thosaikham W, Maneethong S, Sittipout R and Chantiratikul P (2012) Extraction Method for extracting Selenium from Se-enriched Plants. The 8th Mahasarakham University Research Conference, 8th - 9th November 2012, Maha Sarakham, Thailand.
Thosaikham W, Jitmanee K, Chantiratikul A, Sittipout R, Maneethong S and Chantiratikul P. Ion-Paired Reversed Phase HPLC-ICP-MS for Selenium Speciation Analysis in Se-enriched Yeast. Pure and Applied Chemistry International Conference (PACCON 2012). 11 - 13 January 2012, Chiang Mai, Thailand.
Thosaikham W, Jitmanee K, Chantiratikul A, Sittipout R, Manee-thong S and Chantiratikul P, Selenium extraction using mixture of pepsin and cellulase for selenium-enriched Chinese kale (Brassica alboglabra L.H. Bailey). Pure and Applied Chemistry International Conference (PACCON 2010). 21 - 23 January 2010, Ubon Rachathani, Thailand.
Thosaikham W, Sittipout R and Chantiratikul A (2009) Sample preparation methods for selenium determination with ICP-MS technique. The 5th Mahasarakham University Research Conference, 10th - 11th September 2009, Maha Sarakham, Thailand.
Thosaikham W, Chantiratikul P and Chantiratikul A, Determination of plasma selenium content in laying hens fed supplemental sodium selenite or Zinc-L-selenomethionine using ICP-MS Technique. The 3th Mahasarakham University Research Conference, 6 - 7 September 2007, Maha Sarakham, Thailand.
Poster presentation
Thosaikham W, Chantiratikul A, Chantiratikul P (2016) Quantitative of Total Selenium in Selenium-enriched Yeast Products with ICP-MS Using Home-made Closed-digestion System form Sample Preparation. European Symposium on Atomic Spectrometry 2016 (ESAS 2016), 31 March – 2 April 2016, Eger, Hungary.
Thosaikham W, Chantiratikul P, Chantiratikul A, Atiwetin P and Jitmanee K (2009) Development of extraction method for Se-enriched plant preparation. The 6th PERCH-CIC congress. 3 - 6 May 2009, Pattaya, Thailand.
Thosaikham W, Phansi P, Chantiratikul P, Maneechote U and Chantiratikul A (2009) Determination of selenium content in Water meal (Wolffia globosa) supplemental sodium selenate using ICP-MS Technique. Pure and Applied Chemistry International Conference (PACCON 2009). 14 – 16 January 2009, Pitsanolok, Thailand.
Thosaikham W, Chantiratikul P and Chantiratikul A (2007) Quantitative determination of selenium contents in plasma of laying hen using ICP-MS Technique. The Colloquium Spectroscopicum Internationale XXXV, 23 – 27 September 2007, Xiamen, China.
REFERENCES
Asst. Prof. Piyanete Chantiratikul, Ph.D.
Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand
E-mail: [email protected] Tel. +66 871735444
Sujira Maneerat, Ph.D.
Department of biology, Faculty of Science, Mahasarakham University, Thailand
E-mail: [email protected], [email protected] Tel. +66 973199304
Kritsana Jitmanee, Ph.D.
Department of Chemistry, Faculty of Science, Chiang Mai University, Thailand
E-mail: [email protected]