Quantitative analysis of total selenium in of Se-enriched yeast products with ICP-MS using home-made closed digestion system for sample preparation

Authors

  • Witchapol Thosaikham Lecturer

Keywords:

Selenium, closed-digestion system, Se-enriched yeast, water bath, wet acid digestion

Abstract

Sample digestion is an important procedure and directly affects on the reliability of quantitative analysis of total selenium in Se-enriched yeast products. The home-digestion closed digestion system was successfully developed by specially designing and constructing from the basic equipments in laboratory that consisted of 4 main devices; water bath, cover plate, vessels and wind tunnel. The optimum conditions of the home-made closed digestion system was accurately and precisely achieved by using 2.0 ml of nitric acid for 0.1 g Se-enriched yeast sample, 100 °C digestion temperature and 20 min digestion time. These conditions showed good validation and acceptation with the certified value of Se-enriched yeast reference material. Furthermore, the digestion efficiency of home-made closed digestion system was as well as the recognized digestion methods especially microwave assisted digestion. Consequently, the home-made closed digestion system could be efficiently utilized as an alternative digestion method for total Se determination in Se-enriched yeast products. Additionally, the home-made closed digestion system was adapted from a basic instrument in laboratory which has affordability and easier operation rather than the recognized methods.

Author Biography

Witchapol Thosaikham, Lecturer

CURRICULUM VITAE

WITCHAPOL THOSAIKHAM

witphon.tho@msu.ac.th

ORCID ID:  0000-0002-8159-3789

AFILIATION          

Dapartment of Chemistry, Faculty of Science and Technology, Sakon Nakhon Ratjabhat University, Sakon Nakhon 47000

EDUCATION

Ph.D in Chemistry                                                                                                                                                  2016

Mahasarakham University, Thailand

M.Sc. in Chemistry                                                                                                                                                                2010

Mahasarakham University, Thailand

B.Sc. in Chemistry                                                                                                                                                                  2007

Mahasarakham University, Thailand

 

FELLOWSHIP AND AWARS

Research grant for Ph.D. dissertation                                                                                                                      2013-2015

Division of Research Facilitation and Dissemination, Mahasarakham University

Bhumibol Scholarship                                                                                                                                                          2013

Mahasarakham University

Research grant for Master thesis                                                                                                                              2008-2010

Division of Research Facilitation and Dissemination, Mahasarakham University

Scholarship for Master Degree                                                                                                                                  2007-2010

Center of Excellence for Innovation in Chemistry (PERCH-CIC), Thailand

Research grant for Senior Project of the 4th year undergraduate student                                                               2006

Division of Research Facilitation and Dissemination, Mahasarakham University

 

RESEARCH EXPERIENCES 

  • Development of Se-enriched probiotic cultivation and Se speciation analysis 2011 - 2015
  • Design and construction home-made electrodialysis and application to removing 2011 - 2015

        SeO32- ion from remained-medium of Se-enriched probiotics

  • Development of home-made closed vessel digestion and application to preparation                2008 - 2012

         of biological samples prior to determination of trace elements contents by AAS and ICP-MS

  • Se-enriched plant production, Se speciation analysis, and application to poultry diet               2007 – 2010
  • Quantitative determination of selenium contents in plasma of laying hen using               2006 – 2007

ICP-MS Technique      

               

RESEARCH INTERESTS

  • Se-enriched food production and application for producing Se-enriched food with potential to be used in the pharmaceutical or food industry.
  • Development of elemental speciation analysis and metallomics
  • Development of sample introduction system for atomic spectroscopy techniques such as FAAS, HG-AAS, GF-AAS, ICP-OES and ICP-MS
  • Development of sample preparation techniques such as green extraction, digestion and drying
  • Development sample preparation and analytical method for GC, GC-MS and HPLC

 

ANAYTICAL TECHNIQUE SKIL

ICP-OES, ICP-MS, LC-ICP-MS, AAS, HG-AAS, HPLC, GC, UV-Visible spectrometry, Polarimeter, Reflectometer and IR spectrometry

 

WORK EXPERIENCES

      Erawan Sugar Company Limited, Nong Bua Lamphu Province, Thailand                                             2016 - 2017

             - Head of Chemical Analysis Center and Quality Control

Program of Chemistry, Nakhon Ratchasima Rajabhat University, Thailand

             - Special Instructor, Principal Chemistry 1 and Chemistry Laboratory 1,                                        1st semester, 2013

Ministry of Science and Technology of Thailand

- Data Collector, Project Science and Technology Infrastructure Databank (STDB)           April – October 2011

Department of Chemistry, Mahasarakham University, Thailand

- Assistant Teaching, Analytical Chemistry Laboratory 2                                                                              2006 - 2008

- Assistant Teaching, General Chemistry Laboratory 1                                                                                  2007- 2009

- Assistant Teaching, Analytical Chemistry 2, Topic AAS,                                                                  2nd semester, 2015

PATENTS

Thosaikham W, Sujira Maneerat and Chantiratikul P, Process of Se-enriched biomass from Lactobacillus, Thai Patent Application No. 1501004987. Bangkok: Department of Intellectual Property, 2016

Thosaikham W and Chantiratikul P, Electrodialysis Apparatus for Protein Separation, Thai Patent No. 11624. Bangkok: Department of Intellectual Property, 2016

Thosaikham W, Sittipout R and Chantiratikul P (2011) Thai Patent No. 6432. Bangkok: Department of Intellectual Property.

PUBPLICATIONS

Chantiratikul A, Borisuth L, Chinrasri O, Saenthaweesuk N, Chookhampaeng S, Thosaikham W, Sriart N, Chantiratikul P, Evaluation of the toxicity of selenium from hydroponically produced selenium-enriched kale sprout in laying hens, J. Trace Elem. Med. Biol. Vol. 35, pp. 116-121, 2016

10

Thosaikham W, Jitmanee K, Sittipout R, Maneetong S, Chantiratikul A and Chantiratikul P (2014) Evaluation of selenium species in selenium-enriched pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee) using mixed ion-pair reversed phase HPLC-ICP-MS. Food Chemistry, 145, 736-42.

Maneetong S, Chookhampaeng S, Chantiratikul A, Chinrasri O, Thosaikham W, Sittipout R and Chantiratikul P (2013) Hydroponic cultivation of selenium-enriched kale (Brassica oleracea var. alboglabra L.) seedling and speciation of selenium with HPLC–ICP-MS. Microchemical Journal, 108:87-91.

Chantiratikul A, Chinrasri O, Pakmaruek P, Chantiratikul P, Thosaikham W and Sittipout R (2012) Responses of Growing Japanese Quails that Received Selenium from Selenium-Enriched Kale Sprout (Brassica oleracea var. alboglabra L.). Biological Trace Element Research, 144, 760-768.

Chantiratikul A, Chinrasri O, Chantiratikul P, Thosaikham W, Maneethong S and Aengwanich W (2011) Effect of Selenium from Selenium-Enriched Kale Sprout (Brassica oleracea var. alboglabra L.) on Selenium Concentration in Tissue of Spent Hens. Khon Kean Agricultural Journal, 40(2), 295-298.

Chinrasri O, Chantiratikul P, Thosaikham W, Atiwetin P, Chumpawadee S, Saenthaweesuk S and Chantiratikul A (2009) Effect of Selenium-enriched Bean Sprout and Other Selenium Sources on Productivity and Selenium Concentration in Eggs of Laying Hens. Asian-Australasian Journal of Animal Sciences, 22(12), 1661-1666

Thosaikham W and Chantiratikul P (2008) Application of HPLC-ICP-MS technique on elemental speciation analysis. Naresuan Phayou Journal, 3(1), 154-163.

 

PROCEEDING

Thosaikham W, Maneerat S, Chantiratikul P (2017) Evaluation of extracellular selenium speciation in Lactobacillus. Journal of Trace Element in Medicine and Biology, Volume 41S1, p. 61

Chantiratikul P,  Thosaikham W, Maneerat S, (2017) Cultivation of Se-enriched L. casei subsp. rhamnosus for using as Se-enriched diet, Journal of Trace Element in Medicine and Biology, Volume 41S1, p. 60

Borisuth L, Saenthaweesuk N, Chinrasri O, Thosaikham W, Chantiratikul P, Sriart N, and Chantiratikul A (2015) Toxicity of Se from Se-enriched kale sprout (Brassica oleracea var. albogiabla L.) on performance and egg quality in laying hens. Mahasarakham University Science and Technology Journal, Special volume, 270-275.

Thosaikham W, Maneethong S, Sittipout R and Chantiratikul P (2012) Extraction Method for extracting Selenium from Se-enriched Plants. Mahasarakham University Science and Technology Journal, Special volume, 146-151

Sittipout R, Thosaikham W, Chantiratikul P and Burakham R (2011) Development of Home-made Digestion Apparatus for Determination of Iron and Zinc with Flame Atomic Spectrometry Technique in Biological Samples. Mahasarakham University Science and Technology Journal, Special volume, 74-79.

Thosaikham W, Sittipout R and Chantiratikul A (2009) Sample preparation methods for selenium determination with ICP-MS technique. Mahasarakham University Science and Technology Journal. Special volume, 71-78.

 

CONFERENCE

Oral presentation

 

Thosaikham W, Maneerat S, Chantiratikul P. Application of Ion-paired reversed phase HPLC-ICP-MS for Determination of Extracellular Selenium Speciation in Selenium-enriched L. rhamnosus culture. European Symposium on Atomic Spectrometry 2016 (ESAS 2016), 31 March – 2 April 2016, Eger, Hungary

Thosaikham W, Sittipout R and Chantiratikul P (2014) Modification of Water Bath as a Newly Digestion Apparatus and Application for Preparation of Mushroom Samples Prior to Determine Fe Concentration by AAS. Pure and Applied Chemistry International Conference (PACCON 2014). 8 - 10 January 2014,  Khon Kean, Thailand.

Thosaikham W, Maneethong S, Sittipout R and Chantiratikul P (2012) Extraction Method for extracting Selenium from Se-enriched Plants. The 8th Mahasarakham University Research Conference, 8th - 9th November 2012, Maha Sarakham, Thailand.

Thosaikham W, Jitmanee K, Chantiratikul A, Sittipout R, Maneethong S and Chantiratikul P. Ion-Paired Reversed Phase HPLC-ICP-MS for Selenium Speciation Analysis in Se-enriched Yeast. Pure and Applied Chemistry International Conference (PACCON 2012). 11 - 13 January 2012, Chiang Mai, Thailand.

Thosaikham W, Jitmanee K, Chantiratikul A, Sittipout R, Manee-thong S and Chantiratikul P, Selenium extraction using mixture of pepsin and cellulase for selenium-enriched Chinese kale (Brassica alboglabra L.H. Bailey). Pure and Applied Chemistry International Conference (PACCON 2010). 21 - 23 January 2010,                        Ubon Rachathani, Thailand.

Thosaikham W, Sittipout R and Chantiratikul A (2009) Sample preparation methods for selenium determination with ICP-MS technique. The 5th Mahasarakham University Research Conference, 10th - 11th September 2009, Maha Sarakham, Thailand.

Thosaikham W, Chantiratikul P and Chantiratikul A, Determination of plasma selenium content in laying hens fed supplemental sodium selenite or Zinc-L-selenomethionine using ICP-MS Technique. The 3th Mahasarakham University Research Conference, 6 - 7 September 2007, Maha Sarakham, Thailand.

 

Poster presentation

Thosaikham W, Chantiratikul A, Chantiratikul P (2016) Quantitative of Total Selenium in Selenium-enriched Yeast Products with ICP-MS Using Home-made Closed-digestion System form Sample Preparation. European Symposium on Atomic Spectrometry 2016 (ESAS 2016), 31 March – 2 April 2016, Eger, Hungary.

Thosaikham W, Chantiratikul P, Chantiratikul A, Atiwetin P and Jitmanee K (2009) Development of extraction method for Se-enriched plant preparation. The 6th PERCH-CIC congress. 3 - 6 May 2009, Pattaya, Thailand.

Thosaikham W, Phansi P, Chantiratikul P, Maneechote U and Chantiratikul A (2009) Determination of selenium content in Water meal (Wolffia globosa) supplemental sodium selenate using ICP-MS Technique. Pure and Applied Chemistry International Conference (PACCON 2009). 14 – 16 January 2009, Pitsanolok, Thailand.

Thosaikham W, Chantiratikul P and Chantiratikul A (2007) Quantitative determination of selenium contents in plasma of laying hen using ICP-MS Technique. The Colloquium Spectroscopicum Internationale XXXV, 23 – 27 September 2007, Xiamen, China.

 

REFERENCES

Asst. Prof. Piyanete Chantiratikul, Ph.D.

Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand

E-mail: piyanete_s@yahoo.com       Tel. +66 871735444                                                                                         

Sujira Maneerat, Ph.D.

Department of biology, Faculty of Science, Mahasarakham University, Thailand

E-mail: sonkong2000@yahoo.com,  sonkong2000@gmail.com      Tel. +66 973199304

Kritsana Jitmanee, Ph.D.  

Department of Chemistry, Faculty of Science, Chiang Mai University, Thailand

E-mail: k_jitmanee@hotmail.com

Downloads

Published

2018-09-01

How to Cite

Thosaikham, W. (2018). Quantitative analysis of total selenium in of Se-enriched yeast products with ICP-MS using home-made closed digestion system for sample preparation. Creative Science, 10(3), 178–188. Retrieved from https://ph01.tci-thaijo.org/index.php/snru_journal/article/view/106639

Issue

Vol. 10 No. 3 (2018): SNRUJST Vol.10 No. 3 (September - December 2018)

Section

Research Article