Evaluation of Genetic Diversity of Tungat Ali (Eurycoma sp.) Using Simple Sequence Repeat (SSR) Marker
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Abstract
Tongkat Ali (Eurycoma) is a popular medicinal plant in Thailand. Tongkat Ali herb is in the Eurycoma genus originates from the southern border provinces and Malaysia. The Eurycoma is one of the most important genus, however only few comprehensive research studies have dealt on it and methodogies to propagate this genus are also limited. Here, we study to evaluate the genetic diversity of sixteen Eurycoma sp. simple sequence repeat (SSR) markers from southern and northeastern of Thailand in the 18 pairs of microsatellite markers were examined. Authenticated polymorphisms were detected in a total of ten SSR primer pairs and lead to an array of DNA fragments that differed between three band sizes from each fragment, with requiring 1 at least for two states on either side or not. On average, five alleles were recorded for each marker and PIC values varied from 0.26 to 0.68. The genetic similarity coefficients of all samples ranged from 0.63 to 1. Most samples from Narathiwat and Yala provinces were in the same group (similarity coefficient = 0.66), while samples from Amnat Charoen and Ubon Ratchathani provinces were in another group (similarity coefficient = 0.72). This study provides valuable information for establishing a genetic diversity database and guides future studies on active substances in Eurycoma sp., which may contribute to the development of new herbal medicine formulations.
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