Isolation and Molecular Characterization of Antifungal Production from Rice Fields Rhizosphere soil, Thailand

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Tantima Kumlung
Thitiya Rattanakavil
Khanungkan Klanbut

Abstract

One hundred actinomycete strains were isolated from rice field soil Nong Bua, Nakonsawan, Thailand and twenty actinomycete strains were isolated from Happy Rice Organic Farm at Nong sua, Pathumthani, Thailand. A total of one hundred and twenty strains were tested for antifungal activities against rice blast disease (Pyricularia sp. CRI60007). The selected actinomycete strains were chosen from in vitro radial growth inhibition test against Pyricularia sp. CRI60007 that showed the radial growth inhibition percentage of the growth of the fungi (%RGI) more than 50%. The results showed eleven strains gave %RGI more than 50% and strainNK05201 gave the highest %RGI (100 ± 0.00%).Analysis of 16S rRNA gene sequence and blast analyses confirmed that the selected actinomycete,ten strains belong to the genus Streptomyces and one strain belong to the genus Amycolatopsis with similarity to Streptomyces albidoflavus (NK01101(99.56%), NK05201 (99.78%), NK05203 (99.79%), NK05205 (99.79%), NK07201 (99.79%), and NK08202(99.79%)), Streptomyces hydrogenans(NK08203 (100%)), Streptomyces jeddahensis(NK08205 (99.17%)), Streptomyces corchorusii(NK21201 (99.86%)), Streptomyces shenzhenensis(HPF3303 (99.86%)), and Amycolatopsisrhizosphaerae (HPF2102 (99.72%)).

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