Creative Science

Innovative lamellar crystals-based facial treatment cream with Tubtimsiam pomelo peel extract

2023-09-25T14:46:03+07:00

This study examined the antioxidant activity of Tabtimsiam pomelo peel extract (Citrus Maxima Burm.f. Merr) and developed facial cream with lamellar crystals. The results of the analysis total phenolic content with Folin-Ciocalteu reagent assay and total flavonoid content with Aluminium chloride colourimetric assay equal to 48.59 ± 0.66 mg GAE g–1 extract and 184.70 ± 7.04 mg QE g–1 extract, respectively. The result of antioxidants activity with DPPH radical scavenging activity reported by IC50 equal to 63.24 ± 16.57 mg mL–1. The development of facial cream with innovative lamellar crystals compared to base cream found that products containing lamellar crystals more stable than base cream and observed by the visual test with a Microscope under Polarized light found that maltiness cross evenly distributed. Finally, the test of facial cream containing Tubtimsiam pomelo peel extract volume 0.1, 0.2 and 0.3% w w–1 compared with base cream after used 4 weeks found that the highest moisturizing was cream containing extract 0.2% w w–1 equal to 247 ± 1.26 μS and glassy skin when visual skin by Dino-Lite camera.

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HIGHLIGHTS

Structure lamellar crystal of facial treatment cream containing Tubtimsiam pomelo peel extract after used 4 weeks found that the highest moisturizing was cream containing extract 0.2% w w^–¹ equal to 247 ± 1.26 μS and glassy skin

Effect of the drying methods on total phenolics, total flavonoids and antioxidant activities in Ya-Nang leaf (Tiliacora triandra) powder

2023-12-13T08:28:04+07:00

The objective of this research was to study the effect of different drying methods on the amount of total phenolics, flavonoids and antioxidant activity in the Ya-Nang leaf powder using 2 drying methods, were 1) sun drying 2) hot air drying (50 °C 6 h, 60 °C 5 h, 70 °C 4 h) used distilled water as a solvent, then extracted under temperature 80 °C for 45 min. The extract was analyzed for total phenolics content, total flavonoids content and antioxidant capacity measured using DPPH free radical scavenging activity, ABTS free radical scavenging activity, total antioxidant capacity and reducing power. The results found that the drying process at hot air 60 °C for 5 h shown the best condition of total phenolics content (11.277 ± 0.158 mg GAE g–1 powder), total flavonoids content (9.485 ± 0.362 mg CE g–1 powder), DPPH free radical scavenging activity (23.293 ± 0.352 mg AAE g–1 powder), ABTS free radical scavenging activity (10.155 ± 0.393 mg AAE g–1 powder), total antioxidant capacity (32.382 ± 0.414 mg AAE g–1 powder) and reducing power (16.037 ± 0.039 mg AAE g–1 powder). The results indicated the appropriate drying method at 60 °C for 5 h made the Ya-Nang leaf powder contains high bioactive compounds and antioxidant activities. It can use for further development of commercial Ya-Nang tea products.

GRAPHICAL ABSTRACT

HIGHLIGHTS

Preparation of Ya-Nang leaf (Tiliacora triandra) powder by hot air and sun drying
Extraction by distilled water
Analysis of active component and antioxidant activities

Antioxidant activity and Cytotoxicity against the Cervical Epithelial Carcinoma (HeLa) Cell Line of Crude Ganoderma lucidum mycelial extracts

2023-12-06T10:08:44+07:00

This study aimed to investigate the total polysaccharide content, antioxidant activity and cytotoxicity on the Cervical Epithelial Carcinoma (HeLa) cells of the ethanol and water reflux extracts from Ganoderma lucidum mycelia. The phenol-sulfuric acid method was used for the standard method of total polysaccharide analysis, the antioxidant was evaluated by DPPH scavenging assay, ABTS assay, and FRAP assay. The MTT assay was used to determine the cytotoxicity of the extracts. The results showed that the contents of total polysaccharide of the ethanol and water reflux extracts were 8.34 ± 0.47 and 207.81 ± 2.45 mg glucose g extract^–1, respectively. The assays of antioxidant capacity against DPPH and ABTS demonstrated that the water reflux extract showed a strong radical scavenging effect than the ethanol extract. For the reducing capability of both extracts with FRAP method, they showed the reducing antioxidant activity and possessed concentration dependence by which the water reflux extract had a higher FRAP antioxidant activity than the ethanol extract. The cytotoxic assay of the extracts on normal mammalian cells (Vero cells) revealed that the ethanol extract of Ganoderma lucidum mycelia had a cytotoxic effect on normal mammalian cells at 62.5 – 1,000 mg mL^–1, whereas the water reflux extract had no cytotoxicity to normal mammalian cells in all tested concentration. Then, the water reflux extract was selected to determine the cytotoxicity against the HeLa cancer cell line. The results indicated that the water reflux extract of Ganoderma lucidum mycelia exerted a significant cytotoxic effect on HeLa cancer cell line in possessed concentration dependence. The water reflux extract caused a 56.30% decrease in cervical epithelial carcinoma cell viability with concentration of 1 mg mL^–¹. Therefore, the results of this research demonstrated that the extract of Ganoderma lucidum mycelia provide both antioxidant and anti-cancer activities, which could be used as ingredients pharmaceutical products and functional foods.

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Antioxidant activity and Cytotoxicity of Crude Ganoderma lucidum mycelial extracts