@article{Dum-Ampai_Sitthiphrom_Marha_Thakumphu_Anuntalabhochai_Sangwijit_Suebsan_2023, title={Evaluation of genetic variation in longan (Dimocarpus longan Lour) by high annealing temperature random amplified polymorphic DNA (HAT−RAPD)}, volume={15}, url={https://ph01.tci-thaijo.org/index.php/snru_journal/article/view/250017}, DOI={10.55674/cs.v15i2.250017}, abstractNote={<p class="p1"><span class="s1"> Genetic relationships among 20 longan varieties</span> <span class="s2">(<em>Dimocarpus longan</em> Lour.) collected from Chiang Rai</span> and Lampang province; Thailand, Vietnam and China were investigated using high <span class="s2">annealing temperature random amplified polymorphic</span> DNA (HAT<span class="s3">−</span>RAPD) technique. Ten primers, OPA 13, OPAK10, OPB 18, OPG 13, OPW 06, OPW 09, OPX 01, OPX 15, OPZ 01 and OPAK 10, <span class="s1">produced a total of 305 RAPD markers. All primers</span> generated a high degree of polymorphism, ranging in molecular weight from 100 to 2,500 bp. Hierarchical cluster analysis, based on unweighted <span class="s1">neighbor</span><span class="s4">−</span><span class="s1">joining method classified the 20 varieties</span> into 3 major groups. The first two groups could be divided into 2 subgroups and the third group contained only one subgroup. Our data revealed that members in the first group are Daw types. <br />The Narapirom and Petchsakorn varieties exhibited close relatedness, suggesting that they are originated from the same variety. In addition, this technique also detected variation among longan varieties due to selective breeding.</p>}, number={2}, journal={Creative Science}, author={Dum-Ampai, Nattaya and Sitthiphrom, Supranee and Marha, Jenjira and Thakumphu, Boontham and Anuntalabhochai, Somboon and Sangwijit, Kanta and Suebsan, Sugunya}, year={2023}, month={Apr.}, pages={250017} }