@article{Phowichit_Ratanachamnong_Matsathit_Ussawawongaraya_2019, title={Anti-oxidant activity, phenolic and flavonoid constituents of Crude extracts from Piper ribesioides and Zanthoxylum limonella traditional herbal medicine in Northern Thailand}, volume={18}, url={https://ph01.tci-thaijo.org/index.php/rmutt-journal/article/view/164542}, DOI={10.14456/rj-rmutt.2019.3}, abstractNote={<p>The objective of current study was to evaluate an anti-oxidant activity with different solvent polarities (methanol, ethyl acetate, dichloromethane, and hexane) derived from stem of <em>Piper ribesioides</em> together with stalk and seed of <em>Zanthoxylum limonella</em> traditional herbal medicine in Northern Thailand.  Natural antioxidant in medicinal plants were defined for provide antioxidant activity by DPPH scavenging assay together with quantification of the phenolic and flavonoid constituents using Folin Ciocalteau and Aluminium chloride method, respectively.  The polar methanol extract showed greatest antioxidant effect, while ethyl acetate was the second order reaction both in <em>P. ribesioides</em> and <em>Z. limonella</em>.  The ability of methanol extract from stem of <em>P. ribesioides</em> exhibited strongest antioxidant DPPH radical scavenging with IC<sub>50</sub> values as 0.2 mg/ml, which was correlated with maximum value of total phenolic acid (68.83 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.38   mg equivalent of gallic acid per gram, mgGAE/g) and flavonoid content (37.13 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.47 mg equivalent of quercetin per gram, mgQE/g).  Similarly, the highest anti-oxidant activity of <em>Z. limonella</em> stalk and seed extract on methanol extract showed IC<sub>50</sub> values as 0.26 and 0.37 mg/ml, respectively.  Stalk showed higher phenolic content (53.67 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.45 mgGAE/g) than seed (24.15 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.48 mgGAE/g) as well as total flavonoid content of stalk (25.76 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.43 mgQE/g) and seed (10.25 <img title="\pm" src="https://latex.codecogs.com/gif.latex?\pm"> 0.63 mgQE/g).  A relationship between antioxidant activities and increasing levels of total phenolic compounds -or flavonoids was found to be active based on solvent polarities in the extraction of these medicinal plants.  Our findings revealed the preliminary scientific evidence for develop traditional herbal medicine into a valuable source for the future of human health care.</p>}, number={1}, journal={Journal of Applied Research on Science and Technology (JARST)}, author={Phowichit, Suwadee and Ratanachamnong, Piyanee and Matsathit, Udomlak and Ussawawongaraya, Weerasak}, year={2019}, month={Jun.}, pages={25–39} }