@article{Jongjitvimol_Urtgam_2020, title={PHYLOGENETIC AND IDENTIFICATION OF PHITSANULOK’S LANDRACE RICE BASED ON NUCLEOTIDE SEQUENCE ANALYSIS OF TRNH-PSBA}, volume={21}, url={https://ph01.tci-thaijo.org/index.php/psru/article/view/240958}, abstractNote={<p>Landrace rice identification is generally based on morphological characters, which is costly, labor-intensive including time-consuming process. This study was aimed to improve the identification of Phitsanulok’s Landrace rice assay using DNA barcoding based on nucleotide sequence diversity at <em>trnH-psbA </em>locus. The PCR product was amplified corresponding to ~650 bp long which could classify Phitsanulok’s landrace rice into 2 groups. Ton Dip and Lao is the farthest evolution from the ancestor. Individual sequence among 35 landrace-rice cultivars had a high genetic divergence (%K2P) approximately 19%, compared to <em>Oryza sativa</em> L. and 1.3% within group of the Phitsanulok’s landrace rice. Thirty out of Phitsanulok’s landrace rice cultivated varieties was able to be identified by using DNA barcode at <em>trnH-psbA</em> locus with the high efficient of 85.71%. This finding is the first report of implemented DNA barcode at <em>trnH-psbA </em>locus to identify Thai landrace rice’s subspecies using <em>trnH-psbA</em> DNA barcode . However, the combinations of more two DNA barcodes with different loci would be further experimental for increasing efficiency of identification in landrace rice cultivated varieties.</p>}, number={2}, journal={Life Sciences and Environment Journal}, author={Jongjitvimol, Touchkanin and Urtgam, Sittichai}, year={2020}, month={Sep.}, pages={299–310} }