Application of Microbubble Technology for Improved Efficiency and Reduction in Treatment Time of 1-Methylcyclopropene for Postharvest Ripening Delay of Banana

Main Article Content

Hafnee Lateh
Prathan Srichai
Parinya Panich
Prapaipis Tawonsri

Abstract

This research is aimed at evaluating feasibility of using 1-methylcyclopropene microbubbles (1-MCP-MBs) as an alternative postharvest treatment for delaying the ripening of bananas with performance comparison of treating bananas with gaseous fumigation using conventional treatments and without treatment. The effects of immersion time (5, 10, and 15 min), 1-MCP concentration (500, 1000, and 1500 ppb), and microbubble generation pressure (4, 5, and 6 kg/cm2) were tested systematically for 10 days storage period. The results showed the high efficacy of 1-methylcyclopropene microbubbles (1-MCP-MBs) in control of fruit ripening and preventing weight loss when compared with the conventional fumigation with gaseous 1-MCP. The optimal conditions for treating were 1-MCP at 500 ppb, an immersion duration of 5 min, and a generation pressure of 5 kg/cm2. These situations were able to create stable microbubble formation with a high specific surface area resulting in increased adhesion of 1-MCP to the fruit peel. The optimized storage condition resulted in weight loss of 1.68 ± 0.07% during storage for 10 days in comparison to the control weight loss of 3.14 ± 0.56%. Moreover, the treatment using the 1-MCP resulted in a good preservation of the chloroplast integrity and chlorophyll stability, and thus fruit freshness was maintained for over 10 days. Contrastingly, fruits in the control group experienced severe senescing and ripening in 6–8 days. The overall results indicated high potential for 1-MCP-MB technology to be applied as a postharvest technology that can increase competitiveness of banana exports in terms of cost, time, and reduced chemical usage by improved operational efficiency.

Article Details

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Research Article

References

Office of the National Economic and Social Development Council, “Gross domestic product of Thailand: Q3/2025,” Nov. 17, 2025. [Online]. Available: https://www.nesdc.go.th/wordpress/wp-content/uploads/2025/11/BookQGDP-3-2568-Eng.pdf

P. Kulthanavit, “Thailand’s banana exports to Japan,” (in Thai), BU Academic Rev., vol. 23, no. 2, pp. 181–206, 2024.

M. H. Shinga, Y. Silue, and O. A. Fawole, “Recent advancements and trends in postharvest application of edible coatings on bananas: A comprehensive review,” Plants, vol. 14, no. 4, 2025, Art no. 581.

C. Sarpetch, M. Lanchai, P. Sanrucksa, N. Subpakhon, and K. Busarakum, “Efficiency of ethylene absorber paper containing activated carbon from banana sheath in prolonging storage life of ‘Hom Thong’ banana,” (in Thai), Thai J. Sci. Technol., vol. 9, no. 4, pp. 500–511, 2020.

B. M. Abraham, B. K. Saleh, and D. Z. Zelelew, “Effect of post-harvest handling and ripening methods on quality and shelf-life of banana,” Amer. J. Plant Sci., vol. 13, no. 2, pp. 175–192, 2022.

A. Maldonado et al., “Ethylene scavenging films based on ecofriendly plastic materials and nano-TiO2: Preparation, characterization, and in vivo evaluation,” Polymers, vol. 16, no. 6, 2024, Art no. 853.

M. Palumbo et al., “Emerging postharvest technologies to enhance the shelf-life of fruit and vegetables: An overview,” Foods, vol. 11, no. 23, 2022, Art no. 3925.

C. B. Watkins, “The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables,” Biotechnol. Adv., vol. 24, no. 4, pp. 389–409, 2006.

Y. Kartika, T. Srinon, N. Pongprasert, and P. Jitareerat, “Effect of 1-MCP fumigation for preserving the quality of ‘Namwa’ subcluster-isolated bananas for the retail markets,” Agricultural Sci. Innov. J., vol. 55, no. 1 (Suppl.), pp. 109–112, 2024.

T. K. Satekge and L. S. Magwaza, “Postharvest application of 1-methylcyclopropene (1-MCP) on climacteric fruits: Factors affecting efficacy,” Int. J. Fruit Sci., vol. 22, no. 1, pp. 595–607, 2022.

P. Promkaew, S. Kondo, N. Pongprasert, C. Wongs-Aree, S. Kaewsuksaeng, and V. Srilaong, “Application of AVG or 1-MCP-MBs on postharvest quality of Pummelo cv. “Tubtim Siam” (Citrus maxima Burm.),” Food Appl. Bioscience J., vol. 7, no. 3 (Special Issue), pp. 55–71, 2019.

L. P. L. Nguyen, T. Zsom, M. S. Dam, L. Baranyai, and G. Hitka, “Evaluation of the 1-MCP microbubbles treatment for shelf-life extension for melons,” Postharvest Biol. Technol., vol. 150, pp. 89–94, 2019, doi: 10.1016/j.postharvbio.2018.12.017.

A. Patil et al., “Advances in microbubble technologies for food sanitization: A comprehensive review,” Compr. Rev. Food Sci. Food Saf., vol. 24, no. 4, 2025, Art no. e70230.

N. Pongprasert and V. Srilaong, “A novel technique using 1-MCP microbubbles for delaying postharvest ripening of banana fruit,” Postharvest Biol. Technol., vol. 95, pp. 42–45, 2014, doi: 10.1016/j.postharvbio.2014.04.003.

Y. Liu et al., “The molecular regulation of ethylene in fruit ripening,” Small Methods, vol. 4, no. 8, 2020, Art no. 1900485.

B. Cara and J. J. Giovannoni, “Molecular biology of ethylene during tomato fruit development and maturation,” Plant Sci., vol. 175, no. 1–2, pp. 106–113, 2008.

S. Lohani, P. K. Trivedi, and P. Nath, “Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: Effect of 1-MCP, ABA and IAA,” Postharvest Biol. Technol., vol. 31, no. 2, pp.119–126, 2004.

I. Lara, B. Belge, and L. F. Goulao, “The fruit cuticle as a modulator of postharvest quality,” Postharvest Biol. Technol., vol. 87, pp. 103–112, 2014, doi: 10.1016/j.postharvbio.2013.08.012.

R. Alonso-Salinas, S. López-Miranda, A. J. Pérez-López, and J. R. Acosta-Motos, “Strategies to delay ethylene-mediated ripening in climacteric fruits: Implications for shelf life extension and postharvest quality,” Horticulturae, vol. 10, no. 8, 2024, Art no. 840.

N. Pongprasert, S. Phornvillay, P. Lekkham, S. Yodsarn, and V. Srilaong, “1-MCP microbubbles reduce chlorophyll degradation and maintain the quality of Dendrobium cv. Burana Jade orchid flowers,” Scientia Horticulturae, vol. 327, 2024, Art no. 112827, doi: 10.1016/j.scienta.2023.112827.

J. Vera, N. Pongprasert, P. Jitareerat, P. Boonyaritthongchai, and V. Srilaong, “1-MCP microbubbles influence in fruit color and quality of dragon fruit,” Agricultural Sci. J., vol. 48, no. 3 (Suppl.), pp. 237–240, 2017.

H. K. Malahlela, Z. A. Belay, R. R. Mphahlele, and O. J. Caleb, “Micro-nano bubble water technology: Sustainable solution for the postharvest quality and safety management of fresh fruits and vegetables – A review,” Innov. Food Sci. Emerg. Technol., vol. 94, 2024, Art no. 103665, doi: 10.1016/j.ifset.2024.103665.

T. Temesgen, T. T. Bui, M. Han, T.-I. Kim, and H. Park, “Micro and nanobubble technologies as a new horizon for water-treatment techniques: A review,” Adv. Colloid Interface Sci., vol. 246, pp. 40–51, 2017, doi: 10.1016/j.cis.2017.06.011.

J. Yu et al., “High-speed generation of microbubbles with constant cumulative production in a glass capillary microfluidic bubble generator,” Micromachines, vol. 15, no. 6, 2024, Art no. 752.

X. Hu, B. Zhang, C. Wu, X. Xu, M. Xue, and X. Zheng, “Numerical simulation and structural optimization of swirl flow micro-nano bubble generator,” Coatings, vol. 13, no. 8, 2023, Art no. 1468.

Y. Maeda, S. Hosokawa, Y. Baba, A. Tomiyama, and Y. Ito, “Generation mechanism of micro-bubbles in a pressurized dissolution method,” Exp. Therm. Fluid Sci., vol. 60, pp. 201–207, 2015, doi: 10.1016/j.expthermflusci.2014.09.010.

I. G. N. B. Catrawedarma, S. Ton, D. D. Pranowo, and F. Surahmanto, “Hydrodynamic characteristics of the microbubble dissolution in water using an ejector-type bubble generator,” Case Stud. Chem. Environ. Eng., vol. 11, 2025, Art no. 101043, doi: 10.1016/j.cscee.2024.101043.

F. Roshanti, S. D. P. Sidhi, S. Kamal, Deendarlianto, and Indarto, “The performance of venturi microbubble generator type with a 60° twisted baffles,” Eng. Proc., vol. 37, no. 1, 2023, Art no. 116.