Effect of Single Nucleotide Polymorphisms (SNPs) on Microrna-221 on Target Gene in Hepatocellular Carcinoma (HCC) Cell Lines
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Abstract
Hepatocellular carcinoma (HCC) is a disease with a high mortality rate due to the disease is mostly diagnosed in severe phase. The identification of biomarkers is important for early diagnosis of the disease. MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in regulating the expression of many genes and differentially expressed between patients and healthy persons. Moreover, single-nucleotide polymorphisms (SNPs) in miRNAs are affecting on function of miRNAs. This research focused on the effect of SNPs rs113054794 (A/C) within miR-221 for regulation of Aryl hydrocarbon receptor nuclear translocator (ARNT) gene expression in HCC cell line (HepG2). Validation assay based on construction of mi-221 expression vector then transfected into HepG2 cells and measured the expression level of miR-221 by real-time PCR. The result showed that the expression of miR-221 was 65-folds increased in miR-221 transfected group. In addition, the hybridization pattern and the minimum free energy (mfe) between miR-221 and ARNT gene were analyzed by RNAhybrid web-based software. The result revealed that SNPs (A/C) within miR-221 yielded exactly difference in the hybridization pattern and mfe between rs113054794 and ARNT gene (allele C = -25.9 kcal/mol whereas allele A = -18.7 kcal/mol). After that, the expression level of target gene (ARNT) was measured by real-time PCR in order to test the silencing efficiency of miR-221. The result suggested that both alleles of miR-221 triggered ARNT gene silencing. However, ARNT expression level was non-statistically different between allele C and allele A. Therefore, SNPs (A/C) within miR-221 were affected the regulation of ARNT gene expression. However, these SNPs may regulate other genes, which required further study.
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