Developing High-Density Culture System of Pseudodiaptomus annandalei (Copepoda: Calanoida) with Various Microalgae Concentrate

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Pitchaya Chainark
Tawat Sriveerachai
Kom Silapajarn


Pseudodiaptomus annandalei rearing high-density culture system require further development productivity. The feeding copepod with different species of microalgae concentrate could present opportunity of improves production for P. annandalei rearing in high-density culture system. This study was carried out to investigate the population growth (copepod density and specific growth rate) and egg rate (number of female carrying egg) of P. annandalei when fed 3 mono- and 4 combination microalgae concentrate, Chaetoceros sp. (Chae), Isochrysis sp. (Iso), and Nannochloropsis sp. (Nan) Chae+Iso, Chae+Nan, Iso+Nan, and Chae+Iso+Nan in high-density culture system for 10 days. Three replicates, with an initial 1,000 adult P. annandalei per 1 mL (1,250 L fiberglass tank), were set up for each treatment. In all the treatments water temperature and salinity were maintained at 28-29°C and 30-31 ppt. Pure oxygen was used in each treatment in order to maintain adequate dissolved oxygen concentrations under high copepod density culture conditions. The results showed that microalgae concentrate significantly affected population growth. The highest copepod density was 2,820±27 copepod/ mL on P. annandalei fed Chae+Iso+Nan, which was significantly higher (P<0.05) than all other microalgae concentrate tested. The lowest copepod density was 1,520±35 copepod/mL on P. annandalei fed Nan (P<0.05). The highest specific growth rate was 0.29±0.03 of P. annandalei fed Chae+Iso+Nan, and lowest specific growth rate was 0.18±0.02 of P. annandalei fed Nan (P<0.05). Microalgae concentrate also had a significant effect (P<0.05) on egg rate, though the high egg rate was recorded with eggs produced by P. annandalei fed Chae+Iso+Nan (80%), this was not significantly different (P<0.05) from that of eggs produced by P. annandalei fed either Chae+Iso (76%). The results of this study suggest that the combination of Chae+Iso+Nan was the best for the culture of P. annandalei in high-density culture system.


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